Proteomica artículos complementarios 2016

En esta sección del blog encontrarán artículos que podrían interesarles y ayudarles a comprender  diversos temas de proteomica, esta parte del blog se actualizará con base en las recomendaciones de las personas que participan en el curso de proteomica 2016-1.

Simpson DM, Beynon RJ. Acetone precipitation of proteins and the modification of peptides. J Proteome Res. 2010 Jan;9(1):444-50. doi: 10.1021/pr900806x.

Acetone precipitation is a common method for precipitation and concentration of proteins. We show here that a trace amount of residual acetone in the precipitated protein, can, after proteolysis, lead to selective modification of peptides predominantly those in which a glycine residue is the second amino acid, probably generating a relatively stable derivative that, under gas phase conditions, generates a y(1) ion of the same mass as proline. This modification is detectable by either MALDI-ToF or ESI-ion trap mass spectrometry and under normal sample preparation conditions is incomplete. The derivatization occurs in the condensed phase and is sufficiently stable that the modified peptide can elute on reversed phase chromatography at a different time to the unmodified peptide. Acetone precipitation is such a commonly used procedure in protein sample preparation for proteomics that some caution may be warranted. A significant number of peptides (about 5% of a typical proteome) meet the requirements for this reaction and could, therefore, change the outcome of studies.

Ball ZT. Molecular recognition in protein modification with rhodium metallopeptides. Curr Opin Chem Biol. 2015 Apr;25:98-102. doi: 10.1016/j.cbpa.2014.12.017. Epub 2015 Jan 10.

Chemical manipulation of natural, unengineered proteins is a daunting challenge which tests the limits of reaction design. By combining transition-metal or other catalysts with molecular recognition ideas, it is possible to achieve site-selective protein reactivity without the need for engineered recognition sequences or reactive sites. Some recent examples in this area have used ruthenium photocatalysis, pyridine organocatalysis, and rhodium(II) metallocarbene catalysis, indicating that the fundamental ideas provide opportunities for using diverse reactivity on complex protein substrates and in complex cell-like environments.